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Ethidium and propidium monoazide: comparison of potential toxicity on Vibrio sp. viability

Archive ouverte : Article de revue

Copin, S | Mougin, Julia | Raguenet, V | Robert‐pillot, A | Midelet, G | Grard, Thierry | Bonnin‐jusserand, Maryse

Edité par HAL CCSD ; Wiley

International audience. Significance and Impact of the Study: Ethidium monoazide (EMA) and propidium monoazide (PMA) treatment have commonly been used combined with qPCR to discriminate viable and dead cells. However, these DNA-binding dyes can damage membranes in viable bacteria, generating false-negative results. Most available studies examined either EMA-qPCR or PMA-qPCR, based on the literature to select the dye of interest. However, none of them tested the cytotoxicity of EMA vs PMA on various Vibrio sp. strains. In this study, we showed that PMA was the optimal dye for Vibrio as a tool for pretreatment when using qPCR methods to determine Vibrio viability in environmental or seafood samples.

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