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T-DNA insertion mutagenesis

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Sangwan, Rajbir S. | Ochatt, Sergio | Nava-Saucedo, José-Edmundo | Sangwan-Norreel, Brigitte

Edité par HAL CCSD ; CABI Publishing

BAP Geapsi CT1 Chapitre 38. International audience. This chapter focuses on the significance of insertional mutagenesis in functional genomic studies and summarizes T-DNA mutagenesis schemes, including methodologies for creation of T-DNA mutant collections, T-DNA vectors, isolation of the tagged gene and comparisons of the frequency of mutant recovery using various strategies. Concrete examples are also given in cloning genes using T-DNA insertion lines, step by step.

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