Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France

Archive ouverte : Article de revue

Certad, Gabriela | Follet, Jérôme | Gantois, Nausicaa | Hammouma-Ghelboun, Ourida | Guyot, Karine | Benamrouz-Vanneste, Sadia | Frealle, Emilie | Seesao, Yuwalee | Delaire, Baptiste | Creusy, Colette | Even, Gaël | Verrez-Bagnis, Véronique | Ryan, Una | Gay, Mélanie | Aliouat-Denis, Cécile | Viscogliosi, Eric

Edité par HAL CCSD ; Frontiers Media

International audience. Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of Cryptosporidium spp. in sampled fish reached 2.3% (35 out of 1,508) in a first campaign and 3.2% (11 out of 345) in a second campaign. Sequence and phylogenetic analysis of positive samples identified Cryptosporidium parvum (n = 10) and seven genotypes which exhibited between 7.3 and 10.1% genetic distance from C. molnari, with the exception of one genotype which exhibited only 0.5-0.7% genetic distance from C. molnari. Among 31 analyzed fish species, 11 (35.5%) were identified as potential hosts for Cryptosporidium. A higher prevalence of Cryptosporidium spp. was observed in larger fish, in fish collected during the spring-summer period, and in those caught in the North East Atlantic. Pollachius virens (saithe) was the most frequently Cryptosporidium positive species. In fish infected by other parasites, the risk of being Cryptosporidium positive increased 10-fold (OR: 9.95, CI: 2.32–40.01.04, P = 0.0002). Four gp60 subtypes were detected among the C. parvum positive samples: IIaA13G1R1, IIaA15G2R1, IIaA17G2R1, and IIaA18G3R1. These C. parvum subtypes have been previously detected in terrestrial mammals and may constitute an additional source of infection for other animals and in particular for humans. Microscopical examination of histological sections confirmed the presence of round bodies suggestive of the development of C. parvum within digestive glands. We report herein the first epidemiological and molecular data concerning the detection of Cryptosporidium in edible marine fish in European seas surrounding France broadening its host range anduncovering potential novel infection routes.

Consulter en ligne

Suggestions

Du même auteur

First Report on the Prevalence and Subtype Distribution of Blastocystis sp. in Edible Marine Fish and Marine Mammals: A Large Scale-Study Conducted in Atlantic Northeast and on the Coasts of Northern France | Gantois, Nausicaa

First Report on the Prevalence and Subtype Distribution of Blastocystis sp....

Archive ouverte: Article de revue

Gantois, Nausicaa | 2020

International audience. Blastocystis is frequently identified in humans and animal hosts and exhibits a large genetic diversity with the identification of 17 subtypes (STs). Despite its zoonotic potential, its preva...

High association of Cryptosporidium spp. infection with colon adenocarcinoma in Lebanese patients | Osman, Marwan

High association of Cryptosporidium spp. infection with colon adenocarcinom...

Archive ouverte: Article de revue

Osman, Marwan | 2017-12-19

International audience. BackgroundThe association between Cryptosporidium and human colon cancer has been reported in different populations. However, this association has not been well studied. In order to add new s...

Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept | Baydoun, Martha

Three-dimensional (3D) culture of adult murine colon as an in vitro model o...

Archive ouverte: Article de revue

Baydoun, Martha | 2017-12

International audience. Cryptosporidium parvum is a major cause of diarrheal illness and was recently potentially associated with digestive carcinogenesis. Despite its impact on human health, Cryptosporidium pathoge...

Du même sujet

Molecular epidemiology of Cryptosporidium spp. in North Lebanon | Osman, Marwan

Molecular epidemiology of Cryptosporidium spp. in North Lebanon

Archive ouverte: Article de revue

Osman, Marwan | 2018

International audience. Cryptosporidium spp. are enteroparasites with worldwide distribution that infect the gastrointestinal tract of several vertebrates including humans. Human to human, zoonotic, foodborne and wa...

Prevalence and Subtype Distribution of Blastocystis sp. in Senegalese School Children | Khaled, Salma

Prevalence and Subtype Distribution of Blastocystis sp. in Senegalese Schoo...

Archive ouverte: Article de revue

Khaled, Salma | 2020-09-12

International audience. Blastocystis sp. is an enteric protozoan that frequently colonizes humans and many animals. Despite impacting on human health, data on the prevalence and subtype (ST) distribution of Blastocy...

Molecular Characterization of Novel Cryptosporidium Fish Genotypes in Edible Marine Fish | Certad, Gabriela

Molecular Characterization of Novel Cryptosporidium Fish Genotypes in Edibl...

Archive ouverte: Article de revue

Certad, Gabriela | 2020

International audience. Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptos...

Proteolytic activity of Lactobacillus strains isolated from Mongolian traditional dairy products: A multiparametric analysis | Raveschot, Cyril

Proteolytic activity of Lactobacillus strains isolated from Mongolian tradi...

Archive ouverte: Article de revue

Raveschot, Cyril | 2020-01-30

Le Réseau Mixte Technologique Sols et Territoires : développer la connaissance des sols dans les territoires. Le Réseau Mixte Technologique Sols et Territoires : développer la connaissance des sols dans les territoires: Principes, bilan et perspectives | Guellier, Camille

Le Réseau Mixte Technologique Sols et Territoires : développer la connaissa...

Archive ouverte: Article de revue

Guellier, Camille | 2014

absent

Soil bioindicators: how soil properties influence their responses and to select them in function of the site issues? | Pauget, Benjamin

Soil bioindicators: how soil properties influence their responses and to se...

Archive ouverte: Communication dans un congrès

Pauget, Benjamin | 2015-05-03

International audience

Chargement des enrichissements...