Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept

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Baydoun, Martha | Vanneste, Sadia Benamrouz | Creusy, Colette | Guyot, Karine | Gantois, Nausicaa | Chabe, Magali | Delaire, Baptiste | Mouray, Anthony | Baydoun, Atallah | Forzy, Gerard | Chieux, Vincent | Gosset, Pierre | Senez, Vincent | Viscogliosi, Eric | Follet, Jérôme | Certad, Gabriela

Edité par HAL CCSD ; Nature Publishing Group

International audience. Cryptosporidium parvum is a major cause of diarrheal illness and was recently potentially associated with digestive carcinogenesis. Despite its impact on human health, Cryptosporidium pathogenesis remains poorly known, mainly due to the lack of a long-term culture method for this parasite. Thus, the aim of the present study was to develop a three-dimensional (3D) culture model from adult murine colon allowing biological investigations of the host-parasite interactions in an in vivo-like environment and, in particular, the development of parasite-induced neoplasia. Colonic explants were cultured and preserved ex vivo for 35 days and co-culturing was performed with C. parvum. Strikingly, the resulting system allowed the reproduction of neoplastic lesions in vitro at 27 days post-infection (PI), providing new evidence of the role of the parasite in the induction of carcinogenesis. This promising model could facilitate the study of host-pathogen interactions and the investigation of the process involved in Cryptosporidium-induced cell transformation.

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